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B-NDG Kit W41 mice
Strain Name 

NOD.CB17-Prkdcscid Il2rgtm1Bcgen Kittm1(V831M)Bcgen/Bcgen
Common Name 

B-NDG Kit W41 mice

Background

B-NDG

Catalog number  112914
Aliases  Human: PBT, SCFR, C-Kit, CD117, MASTC
NCBI Gene ID
3815

Description



  • C-Kit is expressed in various cells within the body, and it is significantly expressed in stem cells, progenitor cells, and other cells with self-renewal capabilities. In normal bone marrow, c-Kit is expressed by hematopoietic stem cells and plays a crucial role in self-renewal and differentiation into various blood cells. After binding with its ligand SCF, c-Kit undergoes autophosphorylation, thereby activating downstream signaling pathways and participating in cellular processes such as cell proliferation, differentiation, survival, adhesion, motility, and angiogenesis.
  • Gene editing strategy: The 831 acid amino Val of Kit gene was mutated as Met in B-NDG Kit W41 mice. 
  • mRNA expression analysis: Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
  • Application: This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.



mRNA expression analysis


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Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA were isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.


Protein expression analysis-Neutrophils


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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in neutrophils of B-NDG mice and homozygous B-NDG Kit W41 mice. 

Protein expression analysis-DCs

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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in DCs of B-NDG mice and homozygous B-NDG Kit W41 mice. 

Protein expression analysis-Macrophages&Monocytes

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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in CD11b+ cells of B-NDG mice and homozygous B-NDG Kit W41 mice. 

Frequency of leukocyte subpopulations

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Frequency of leukocyte subpopulations by flow cytometry. Splenocytes, blood cells and bone marrow cells were isolated from B-NDG mice and homozygous B-NDG Kit W41 mice (n=3, 8-week-old). Flow cytometry analysis of the Splenocytes, blood cells and Bone marrow cells were performed to assess the frequency of leukocyte subpopulations. Percentages of T cells, B cells, NK cells, DCs, granulocytes, monocytes, macrophages in B-NDG Kit W41 mice were similar to those in B-NDG mice, demonstrating that mutation of Kit does not change the frequency or distribution of these cell types in spleen(A), blood(B) and bone marrow(C). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001.